SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Comparaison de methodes directes (microscopiques) et indirectes (par mise en culture) dans l'evaluation d'une pollution bacterienne d'origine fecale. Etude preliminaire

Heral, Maurice; Prou, Jean.

A seasonal study of total microflore shows that heterotrophic germs are submitted to a distinctive evolution in autumn and winter suspension and degradation of detritic material; in spring an summer bacterial growth due to degradation of phytoplanction. In summer enterocoques give evidence of positive summer pollution. Survival on the mud of Escherichia coli and overall of enterocoques indicates that the substration is particularly suitable to germ proliferation;

Tipo: Text

Palavras-chave: Marennes Oléron; Escherichia coli; Bactériologie; Biomasse.

Ano: 1980

URL: http://archimer.ifremer.fr/doc/1980/acte-3005.pdf

The behaviour of F specific bacteriophage in depurating shellfish with reference to their use as pollution indicator organisms

Munro, P; Bianchi, M.

Cells of two serotypes of Escherichia coli (O26B6 and 0111B4) were counted using epifluorescence and immunofluorescence microscopy (nonavalent serum) and plate count on specific medium. Values from indirect counts were 10 fold smaller than those from both microscopic counts. In a sample of sea water from an area receiving a fecal pollution, the highest number of bacteria was obtained by epifluorescence microscopy. In that case, the number of cells counted by immunofluorescence microscopy was 10 fold smaller than the number of bacteria able to grow on the medium. It could be due to: the diversity of the natural community, the possibility to other bacteria than E. coli to grow on the used culture medium, the restrictiveness of the serum for "pathogenic...

Tipo: Text

Palavras-chave: Escherichia coli; Bacteria; Microbiological culture; Pathogenic bacteria; Microbial contamination; Bacterial counters; Fluorescence microscopy; Bacteria collecting devices; Indicator species.

Ano: 1984

URL: http://archimer.ifremer.fr/doc/1984/acte-1001.pdf

Traitement de l''eau par ultraviolets, application a la purification des coquillages

Lees, D; Dore, W.

The behaviour of sex-pilli specific (F+) bacteriophage during the depuration process was investigated using Pacific oysters (Crassostrea gigas) and edible mussels (Mytilus edulis) naturally contaminated with untreated sewage. F+ bacteriophage was eliminated considerably slower than E. coli. This effect was most pronounced in oysters which showed average T90 bacteriophage values of 62.5 hours compared with 11 hours for E. coli. F+ bacteriophage in mussels was largely confined to the digestive tract. The majority of E. coli were similarly located but, in contrast, counts were also distributed throughout the other internal tissues. Investigation of ultra-violet (UV) dosage showed that the difference in depuration rate was unlikely to be due to the higher...

Tipo: Text

Palavras-chave: Escherichia coli; Mytilus edulis; Crassostrea gigas; Bivalvia; Indicator species; Marine molluscs; Bacteriophages; Pollution indicators; Marine pollution; Shellfish; Self purification.

Ano: 1992

URL: http://archimer.ifremer.fr/doc/1992/acte-1605.pdf

Bilan des analyses du réseau microbiologique (REMI), en Languedoc-Roussillon, d'avril 1989 à décembre 1991

Cailleres, J.

The germicide properties of ultra-violet radiation in a wave length ranging from 100 to 400 mm were already discovered back in the late 19th century. These properties specific to UV-C radiation (200-280 mm) result from its action on the nucleic acids of the micro-organisms. Each micro-organism, depending on its composition, therefore presents a specific sensitivity to this radiation.

Tipo: Text

Palavras-chave: France; Escherichia coli; Crassostrea; Bivalvia; Bacteria; Ultraviolet radiation; Self purification; Shellfish culture.

Ano: 1992

URL: http://archimer.ifremer.fr/doc/1992/acte-1629.pdf

Comps, Marie-annick; Tournier, Henri; Sospedra, Colette.

Le réseau de surveillance microbiologique d'IFREMER a été mis en place dans toute la France en 1989, dans le but de garantir la santé publique et de suivre la qualité de l'environnement. Dans la région Languedoc-Roussillon sept sites producteurs de coquillages sont surveillés chaque mois ou plus fréquemment si· nécessaire. Dans le présent rapport, les méthodes d'analyses et les critères d'étude des données sont décrits; les résultats acquis d'avril 1989 à décembre 1991 sont présentés pour chaque site en essayant d'expliquer les contaminations observées par les sources de pollution potentielles recensées, la pluviométrie ou tout autre facteur local. Un traitement des données conduit à différentes conclusions : - En mer, où l'élevage des moules sur filière...

Tipo: Text

Palavras-chave: Réseau microbiologique; Languedoc-Roussillon; Escherichia coli; Salmonelles; Surveillance.

Ano: 1993

URL: http://archimer.ifremer.fr/doc/00418/52976/53904.pdf

DIARRÉIA NEONATAL DE ORIGEM BACTERIANA EM BOVINOS

Pianta,Celso.

Diarréia neonatal em bovinos é um quadro complexo, normalmente responsável por altas taxas de morbidade e mortalidade. Os prejuízos são observados não somente nos gastos com a medicação, mas também na perda do animal em caso de morte e na redução do desenvolvimento e do desempenho do futuro animal adulto, comprometendo os índices de ganho de peso ou produção leiteira. A etiologia múltipla da enfermidade envolve a interação de diversas variáveis dos agentes, hospedeiro e meio ambiente. Nesta revisão são abordados os aspectos clínicos da colibacilose sanitário, a prevenção e o tratamento são também abordados.

Prevalence of enteropathogenic and enterotoxigenic Escherichia Coli in foods of animal origin in southern Brazil

Palavras-chave: Diarréia neonatal; Escherichia coli; Bezerros.

Ano: 1993

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781993000100021

Aleixo,José Antonio; Aver,Gladis Paim.

Prevalence of Escherichia coli in foods of animal origin from the city of Pelotas, Brazil, was determined. The ocurrence of enterotoxigenic (ETEC) and classic enteropathogenic (EPEC) serogroups among E. coli isolates was determined. E. coli was isolated from 68% of the food samples surveyed. Of 36 food samples tested, 11(30%) and 24(66%) were positive for EPEC and ETEC strains respectively. However, of 187 E. coli isolates tested, 30(16%) were EPEC compared to 75(40%) which were ETEC. The antibiotic resistance pattern revealed that the isolates were highly sensitive to all antibiotics tested.

POPULATION ANALYSIS OF SUSCEPTIBILITY TO PROPOLIS IN REFERENCE STRAINS OF Staphylococcus aureus AND Escherichia coli

Palavras-chave: Escherichia coli; EPEC; ETEC; Foods; Brazil.

Ano: 1996

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781996000200013

J. Venom. Anim. Toxins

FERNANDES Jr.,A.; LOPES,C. A. M.; SFORCIN,J. M.; FUNARI,S.R.C..

Susceptibility to several ethanolic extracts of propolis (EEP) concentrations was tested with the population analysis technique in reference strains of Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 35218). The results of these tests showed that all bacteria were killed by EEP concentrations approximately equal or higher (2.0% v/v- S. aureus; 10.0% v/v - E. coli) than the respective minimal inhibitory concentrations (MIC). Regarding the susceptibility to propolis, there was a homogeneity of data with the respective time kill curves showing a clear bactericidal effect during 6 to 9 h of exposition.

Adaptative responses of E-coli to marine environmental stresses. a modelling approach based on viability and dormancy concepts

Palavras-chave: Propolis; Population analysis; Susceptibility; Staphylococcus aureus; Escherichia coli.

Ano: 1997

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301997000200005

ANTAGONISM AGAINST VIBRIO CHOLERAE BY BACTERIAL DIFFUSIBLE COMPOUND IN THE FECAL MICROBIOTA OF RODENTS

Martin, Y; Troussellier, Marc; Bonnefont, Jl.

In an attempt to synthesize conceptual and experimental information on the behaviour of enteric bacteria in seawater, a mathematical model has been developed. This model is based on changes in intracellular metabolisable components depending an physiological responses of the cell when subjected to nutrient starvation, salinity stress and solar radiation. Following a strategy which takes into account short- and mid-term physiological adaptation and reversal processes, the cells can develop in different states: culturable (B-1), viable and definitively nonculturable (B-2), and reversibly dormant (B-3) cells. Model parameters were deduced either from original microcosm experiments, from literature data or calibration procedures. Validation of the model was...

Tipo: Text

Palavras-chave: Escherichia coli; Eau de mer; Stress; États cellulaires; Modélisation; Escherichia coli; Seawater; Stress; Cellular states; Modelling.

Ano: 1998

URL: http://archimer.ifremer.fr/doc/00325/43646/43224.pdf

Rev. Microbiol.

Silva,Simone Helena da; Vieira,Enio Cardillo; Nicoli,Jacques Robert.

In an ex vivo agar plate assay, we monitored the appearance of an inhibitory halo against Vibrio cholerae from the feces of Wistar and Fischer rats aged 10 to 42 days. The frequency of Wistar rats showing halo increased from 0% (10 days) to a maximum of 80.0% (29 days) and then decreased to 53.3% (42 days). A similar pattern was obtained with Fischer rats but with a lower intensity (maximum frequency of 50.0% by day 36). In a separate experiment, when Wistar rats were fed a low-protein diet for 7 days, the inhibitory halo decreased drastically. Three apparently different colony morphologies were isolated from the dominant fecal microbiota: a facultative anaerobe (FAN) and two strict anaerobes (SAN). The ex vivo inhibitory test showed a halo around the...

Spontaneous kanamycin-resistant Escherichia coli mutant with altered periplasmic oligopeptide permease protein (OppA) and impermeability to aminoglycosides

Palavras-chave: Vibrio cholerae; Escherichia coli; Streptococcus intermedius; Antagonism; Rodents.

Ano: 1998

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000300016

Rev. Microbiol.

Rodriguez,Mônica B.; Costa,Sérgio O. P..

A spontaneous kanamycin-resistant Escherichia coli mutant, showing cross resistance to five other aminoglycosides and absence of the OppA protein was isolated. [3H]-dihydrostreptomycin uptake is reduced in this mutant, implying that the oligopeptide transport system is involved in accumulation of aminoglycosides, although apparently not related with aminoglycoside permeability alteration due to bacterial adaptation to osmotic changes.

Tipo: Info:eu-repo/semantics/other

Palavras-chave: Aminoglycosides-resistance; Escherichia coli; OppA.

Ano: 1999

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200013

Avaliação de ensaios analíticos para detecção de coliformes fecais em queijo Minas

Arq. Bras. Med. Vet. Zootec.

Pereira,M.L.; Gastelois,M.C.A.; Bastos,E.M.A.F.; Caiaffa,W.T.; Faleiro,E.S.C..

Foram submetidas à pesquisa de coliformes fecais, utilizando-se a técnica do número mais provável (NMP/g), 168 amostras de variedades de queijo Minas (20 frescal, 48 canastra e 100 padronizado) coletadas em Belo Horizonte. Para a comparação de diferentes ensaios em temperatura elevada, utilizou-se o caldo EC isoladamente, e caldos EC e triptofano em paralelo. Visando à pesquisa de indol foi realizado ensaio para confirmação de produção de beta-D-glucuronidase e indol em caldo fluorocult lauril sulfato. Os resultados demonstraram não haver diferença estatística significativa entre as três metodologias utilizadas para a pesquisa de coliformes fecais, considerando os índices de aceitação definidos pelos padrões legais de inspeção de queijo Minas. A facilidade...

Experimental peritonitis in horses: peritoneal fluid composition

Palavras-chave: Queijo Minas; Coliformes fecais; Escherichia coli.

Ano: 1999

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000500004

Arq. Bras. Med. Vet. Zootec.

Mendes,L.C.N.; Marques,L.C.; Bechara,G.H.; Peiró,J.R..

Sixteen adult horses were randomly divided into four equal groups of four animals and each group was injected intraperitoneally with one of the following suspension: Group I, 100×10(7) colony-forming units (CFU) of E. coli diluted in 500ml of 0.9% saline; Group II, 100×10(7) CFU of Bacteroides fragilis in 500ml of 0.9% saline; Group III, 100×10(7) CFU of E. coli in combination with 100×10(7) CFU of B. fragilis in 500ml of 0.9% saline; Group IV, 500ml of 0.9% saline. A significant increase in leukocyte number was observed in the peritoneal fluid by four hours after the inoculations in animals of Group I and II, and by eight hours in animals of Group III. The highest cell count observed was 516×10³ leukocytes/mm³. Significant increases in peritoneal fluid...

Clinical aspects of experimental peritonitis in horses

Palavras-chave: Horses; Peritonitis; Escherichia coli; Bacteroides fragilis.

Ano: 1999

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000300003

Mendes,Luiz Cláudio Nogueira; Marques,Luiz Carlos; Iturrino,Ruben Pablo Schocken; Ávila,Fernando Antônio de.

Sixteen adult horses were randomly divided into 4 equal groups (GI, GII, GIII and GIV) of 4 animals and each group was injected intraperitoneally with one of the following suspension: GI (100 x 10(7) colony-forming units (CFU) of Escherichia coli diluted in 500ml of 0.9% saline); GII (100 x 10(7) CFU of Bacteroides fragilis in 500ml of 0.9% saline); GIII (100 x 10(7) CFU of E. coli in combination with 100 x 10(7) CFU of B. fragilis in 500ml of 0.9% saline); GIV (500ml of 0.9% saline). Abdominal wall sensitivity to external pressure and tension, diarrhea, decreased intestinal sounds and increased of heart rate were the clinical signs more frequently observed in inoculated horses. Horses inoculated with pure cultures of either E. coli or B. fragilis...

Avaliação da eficiência de um probiótico no controle de diarréia e no ganho de peso de bezerros

Palavras-chave: Peritonitis; Horses; Escherichia coli; Bacteroides fragilis.

Ano: 1999

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781999000300019

Arq. Bras. Med. Vet. Zootec.

Ávila,F.A.; Paulillo,A.C.; Schocken-Iturrino,R.P.; Lucas,F.A.; Orgaz,A.; Quintana,J.L..

Foram utilizadas 99 vacas prenhes distribuídas em oito grupos que receberam os seguintes tratamentos: grupo I, com 29 vacas não vacinadas e seus bezerros que não receberam probiótico, ficando como controle; grupo II, com 10 vacas vacinadas e seus bezerros que não receberam probiótico; grupos III, IV e V, com 10 animais cada, vacas vacinadas e seus bezerros que receberam probiótico durante 5, 15 e 30 dias, respectivamente; os grupos VI, VII e VIII, com 10 animais cada, vacas não vacinadas e seus bezerros que receberam probiótico durante 5, 15 e 30 dias, respectivamente. Cada animal dos grupos vacinados recebeu duas doses vacinais contendo os pili K99 e A14 de Escherichia coli na dose de 5,0ml por via subcutânea. O probiótico contendo Ruminobacter...

Experimental peritonitis in horses. Hematological and biochemistry aspects

Palavras-chave: Bovino; Escherichia coli; Probiótico; Diarréia.

Ano: 2000

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000100011

Braz. J. Vet. Res. Anim. Sci.

MENDES,Luiz Cláudio Nogueira; MARQUES,Luiz Carlos; SCHOCKEN-ITURRINO,Ruben Pablo; ÁVILA,Fernando Antônio de; MALHEIROS,Euclides Braga.

Sixteen adult horses were randomly divided into 4 equal groups (GI, GII, GIII and GIV) of 4 animals and each group was injected intraperitoneally with one of the following suspension: Group I, 100 x 10(7) colony-forming units (CFU) of E. coli diluted in 500 ml of 0.9% saline; Group II, 100 x 10(7) CFU of Bacteroides fragilis in 500 ml of 0.9% saline; Group III, 100 x 10(7) CFU of E. coli in combination with 100 x 10(7) CFU of B. fragilis in 500 ml of 0.9% saline; Group IV, 500 ml of 0.9% saline. Leukopenia appeared in all animals inoculated with bacteria within the first six hours of the experiment. After this period, leukocytosis was observed in some inoculated horses. Horses inoculated with pure cultures of either E. coli or B. fragilis demonstrated mild...

Fermentation of sweet whey by recombinant Escherichia coli K011

Palavras-chave: Peritonitis; Horses; Escherichia coli; Bacteroides fragilis.

Ano: 2000

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000200011

BJM

Leite,Amarildo Ricardo; Guimarães,Walter Vieira; Araújo,Elza Fernandes de; Silva,Daison Olzany.

The production of ethanol from sweet whey using the recombinant Escherichia coli KO11, in batch fermentation, was tested. The maximum ethanol yield was reached after 96h, representing only 38% of the theoretical yield. The supplementation of whey with components of LB broth increased the maximum yield to 96% in 72h. The addition of 0.5% yeast extract to whey resulted in maximum yield of 74% at 36h and it increased to over 100% when yeast extract and trace metals solution (Fe++, Mn++ and Zn++) were added.

Motilidade espermática e integridade acrossomal em doses de sêmen suíno refrigeradas e inoculadas com Esecherichia coli e Staphylococcus aureus

Palavras-chave: Escherichia coli; Sweet whey; Ethanol.

Ano: 2000

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300011

Bennemann,Paulo Eduardo; Bortolozzo,Fernando Pandolfo; Wentz,Ivo; Cardoso,Marisa Ribeiro de Itapema.

Foram utilizados 12 ejaculados, coletados de maneira asséptica, distribuídos em sete tratamentos, sendo um grupo controle e os demais inoculados com três diferentes concentrações de S. aureus ou E. coli (5 x 10(5), 5 x 10(6) e 5 x 10(7) UFC/ml). Durante 96 horas, foram avaliados a motilidade espermática, o percentual de acrossomas intactos (NAR), o pH e o número de unidades formadoras de colônia (UFC/ml) das bactérias inoculadas. O desenvolvimento bacteriano foi decrescente ao longo das 96 horas de armazenamento. Com exceção do tratamento com a inoculação de 5 x 10(7) UFC de E. coli/ml, não foi observado efeito significativo das bactérias sobre a motilidade espermática (p>0,05). Da mesma forma, não houve efeito significativo (p>0,05) do S. aureus ou...

Validity of Escherichia coli, enterovirus, and F-specific RNA bacteriophages as indicators of viral shellfish contamination

Palavras-chave: Sêmen; Acrossoma; Escherichia coli; Staphylococcus aureus.

Ano: 2000

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782000000200019

Miossec, Laurence; Le Guyader, Soizick; Pelletier, Dominique; Haugarreau, Larissa; Caprais, Marie-paule; Pommepuy, Monique.

The sanitary classification of harvesting areas for bivalve mollusks in France is based on the level of Escherichia Coli contamination detected in shellfish meat, as defined in EC Directive 91/492 EEC. However, outbreaks of gastroenteritis or hepatitis after consumption of shellfish meeting current bacteriological standards suggest that E. coli is a poor indicator of viral contamination. The purpose of this study was to assess the adequacy of enterovirus and F-specific RNA bacteriophages as new indicators of human enteric viruses. Shellfish were sampled over a 37-mo period to characterize microbial contamination in two coastal areas subjected to different sewage contamination inputs. Contamination by E. coli, F-specific RNA bacteriophages (F+ RNA) and...

Tipo: Text

Palavras-chave: Indicator; Escherichia coli; F specific RNA bacteriophages; Enteric viruses; Viral contamination; Shellfish.

Ano: 2001

URL: https://archimer.ifremer.fr/doc/00000/10006/7794.pdf

Characterization of a highly thermostable alkaline phosphatase from the euryarchaeon Pyrococcus abyssi